Genetic resources and biotechnology : Consultant final report IICA/EMBRAPA-PROCENSUL II
Por: Maruniak, J.E [autor/a]
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Colaborador(es): IICA, Brasilia, DF (Brasil) | Empresa Brasileira de Investigación Agropecuaria (EMBRAPA) Brasilia, Brasil.
Tipo de material: 
ArtículoSeries Publicações Miscelâneas A4-BR (IICA) ; no. 89-044. Editor: Brasilia, DF (Brasil): IICA, 1989Descripción: 9 páginas.ISSN: 0534-0591.Tema(s): RECURSOS GENETICOS | BIOTECNOLOGIA ANIMAL | ANTICARSIA | BACULOVIRIDAE | CONTROL BIOLOGICORecursos en línea: eng Resumen: Research activities developed: 1) a rapid microextraction protocol for baculovirus DNA from either infected cell cultures or infected larvae was demonstrated. 2) Anticarsia baculovirus infected Anticarsia larvae brought from EMBRAPA Soja were tested for the effectiveness of the rapid microextraction protocol in purifying viral DNA. 3) Restriction enzyme analysis of microextracted viral DNA and gel electrophoresis were performed. 4) Reagents, cell culture supplies and titered Anticarsia baculovirus were brought to perform infected insect cell protein synthesis experiments. 5) Attempts were made to modify the rapid microextraction protocol to purify fungal mitochondrial DNA by using lysozyme to make fungal protoplasts before superating mitochondrial DNA from chromosomal DNA. (MIBA)
| Tipo de ítem | Ubicación actual | Colección | Signatura | Estado | Fecha de vencimiento | Código de barras |
|---|---|---|---|---|---|---|
Documento impreso
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Biblioteca Conmemorativa Orton | Colección IICA | IICA PM-A4/BR 89-044 (Navegar estantería) | Disponible | 82729 | |
Documento digital
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Sede Central | Colección IICA | IICA-PM A4/BR No.89-044 (Navegar estantería) | Disponible | BVE20108200 |
Research activities developed: 1) a rapid microextraction protocol for baculovirus DNA from either infected cell cultures or infected larvae was demonstrated. 2) Anticarsia baculovirus infected Anticarsia larvae brought from EMBRAPA Soja were tested for the effectiveness of the rapid microextraction protocol in purifying viral DNA. 3) Restriction enzyme analysis of microextracted viral DNA and gel electrophoresis were performed. 4) Reagents, cell culture supplies and titered Anticarsia baculovirus were brought to perform infected insect cell protein synthesis experiments. 5) Attempts were made to modify the rapid microextraction protocol to purify fungal mitochondrial DNA by using lysozyme to make fungal protoplasts before superating mitochondrial DNA from chromosomal DNA. (MIBA)
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